Research Articles
Beyond Microscopy: Advancing Entamoeba histolytica Diagnosis with High-Specificity Antigen Tests
Accurate differentiation of pathogenic Entamoeba histolytica from non-pathogenic Entamoeba dispar is critical for appropriate treatment and avoiding undue therapy.
Commercial vs. In-House PCR Assays: A Comprehensive Analysis of Clinical Sensitivity and Diagnostic Utility
This article provides a critical comparison of the clinical sensitivity of commercial PCR kits and laboratory-developed in-house assays, tailored for researchers and drug development professionals.
Strategic Primer Optimization for Accurate Entamoeba histolytica and dispar Differentiation in Research and Diagnostics
Accurate differentiation of the morphologically identical Entamoeba histolytica and Entamoeba dispar is critical for amoebiasis research, drug development, and patient management, as one is a pathogen and the other is...
Breaking the Barrier: Strategies to Maximize DNA Yield from Resilient Cryptosporidium Oocysts
The robust, multi-layered wall of the Cryptosporidium oocyst is a significant obstacle for molecular diagnostics and research, as it severely limits DNA extraction efficiency.
Pediatric Giardiasis: Epidemiology, Transmission Routes, and Intervention Strategies
This article provides a comprehensive analysis of the risk factors and transmission dynamics of Giardia duodenalis in children, a leading cause of parasitic gastrointestinal disease worldwide.
Molecular Epidemiology of Cryptosporidium hominis and parvum: Trends, Transmission, and Typing Tools
This article synthesizes current knowledge on the molecular epidemiology of Cryptosporidium hominis and C.
Microscopy vs. Molecular Methods for Protozoan Diagnosis: A Critical Comparison for Modern Laboratories
This article provides a comprehensive analysis for researchers, scientists, and drug development professionals on the evolving landscape of protozoan parasite diagnostics.
The Critical Signal in the Silence: Leveraging Negative Results in Wildlife Disease Surveillance for Global Health Security
This article addresses the critical yet often overlooked role of negative results in wildlife disease surveillance, a cornerstone for accurate epidemiology and effective global health security.
Conquering Heterogeneity: Advanced Spatial and Temporal Sampling Strategies for Precision Parasitology
Spatial and temporal heterogeneity in parasite distribution fundamentally challenges effective disease control, drug development, and elimination efforts.
Advanced Environmental Sampling for Soil-Transmitted Helminths: Methodologies, Optimization, and Validation for Biomedical Research
This article provides a comprehensive guide to environmental sampling for soil-transmitted helminth (STH) stages, addressing key challenges in transmission hotspot identification and surveillance.