Research Articles
Overcoming False Negatives in Dientamoeba fragilis PCR: A Research-Focused Guide to Enhanced Detection and Assay Validation
Molecular diagnostics, particularly PCR, have revolutionized the detection of Dientamoeba fragilis but are susceptible to false-negative results that can impede research and drug development.
Optimizing Primer Templates for Trichomonad Detection: A Strategic Guide for Molecular Assay Development
Accurate detection of Trichomonas vaginalis is critical for addressing the global burden of this prevalent non-viral sexually transmitted infection.
Advancing Cryptosporidium Detection: Strategies for Enhanced Sensitivity in Clinical and Research Settings
Cryptosporidium, a significant cause of diarrheal disease, has been historically underestimated due to the insensitivity of conventional diagnostic methods.
Overcoming PCR Inhibition in Stool Samples: A Comprehensive Guide for Robust Molecular Diagnostics
Accurate molecular analysis of stool samples is critical for clinical diagnostics, gut microbiome research, and drug development.
Breaking the Wall: Advanced Strategies for DNA Extraction from Robust Parasite Oocysts
Efficient DNA extraction from robust parasite oocysts, such as Cryptosporidium and Giardia, is a critical bottleneck in molecular diagnostics and research.
FEA Stool Concentration: Enhanced Detection of Intestinal Parasites in Clinical and Research Settings
This article provides a comprehensive analysis of the Formalin-Ethyl Acetate (FEA) concentration technique for stool specimen examination, a critical diagnostic tool for intestinal parasitic infections (IPIs).
Multi-Amplicon Sequencing for Parasite Load Assessment: A Comprehensive Guide for Surveillance and Drug Development
Multi-amplicon sequencing is revolutionizing parasite load assessment and molecular surveillance by enabling highly sensitive, parallel analysis of multiple genomic targets.
Automated Nucleic Acid Extraction from Stool Samples: A Comprehensive Guide for Researchers and Developers
Automated nucleic acid extraction from stool samples is a critical yet challenging step in molecular diagnostics and microbiome research.
Universal Parasite Diagnostic (nUPDx): A Deep-Amplicon Sequencing Framework for High-Sensitivity Detection of Blood-Borne Parasites
This article explores the nested Universal Parasite Diagnostic (nUPDx), a targeted amplicon deep sequencing (TADS) approach that uses the 18S rDNA gene and selective restriction enzyme digestion to detect and...
Harnessing AI-Powered Image Analysis: A New Paradigm in Parasitic Disease Diagnostics and Drug Discovery
This article explores the transformative role of Artificial Intelligence in parasitic disease control, addressing a critical need for researchers, scientists, and drug development professionals.