Hidden World of Avian Blood Parasites: The Sri Lankan Story

Beneath the vibrant plumage and melodious songs of Sri Lanka's diverse bird communities lies a hidden world of conflict and survival

Introduction

Across the globe, avian blood parasites silently shape bird populations, influencing their health, behavior, and evolution. In tropical Sri Lanka, where biodiversity thrives in its lush landscapes, scientists are unraveling the complex relationships between native birds, their parasitic inhabitants, and the mosquito vectors that transfer these microscopic organisms.

Bird Diversity

Sri Lanka hosts numerous endemic and migratory bird species that serve as hosts for blood parasites.

Hidden Parasites

Microscopic parasites silently influence bird health and population dynamics.

Avian Blood Parasites: An Unseen World

Avian haemosporidian parasites are single-celled organisms that infect birds' blood cells, with three main genera causing most infections: Haemoproteus, Plasmodium, and Leucocytozoon.

Parasite Life Cycle
Infection

Mosquito bites an infected bird, ingesting parasites with blood

Development

Parasites undergo development within the insect vector

Transmission

Infected mosquito transmits parasites to new bird host during feeding

Parasite Impact
High Mortality
During acute infection phases
Reduced Reproduction
Lower reproductive success
Shorter Lifespan
Affected by chronic infections

Sri Lanka's Avian Blood Parasite Landscape

In a comprehensive study of Sri Lankan bird communities, researchers made remarkable discoveries about the prevalence and diversity of these hidden organisms.

Parasite Distribution
Blood Parasites Detected in Sri Lankan Birds
Parasite Genus Species Identified Example Host Species
Haemoproteus 4 Yellow-browed bulbul, Oriental white-eye
Plasmodium 5 Red-vented bulbul, Asian Brown flycatcher

The study found infections in seven different bird species, including four resident and three migratory species 2 .

Key Finding: Approximately 85% of the detected Plasmodium and Haemoproteus sequences did not match well-characterized species from other regions, suggesting Sri Lanka may host distinct parasite lineages 2 .

Mosquito Vectors: The Bridge of Infection

No blood parasite can complete its life cycle without its insect vectors. In Sri Lanka, researchers extended their investigation beyond birds to examine potential mosquito vectors.

Sample Collection

480 mosquito samples collected from various locations including Halgolla forest reserve and forest patches in Kurunegala and Gampola 2 .

Positive Findings

Two mosquito species tested positive—three for Plasmodium and one for Haemoproteus 2 .

New Discoveries

First report of Culex cinctellus in Sri Lanka, detected using bird-baited traps 8 .

Transmission Cycle
Infected Bird
Mosquito Vector
New Bird Host

Scientific Toolkit: Uncovering Hidden Parasites

Detecting these microscopic parasites requires sophisticated molecular techniques.

Research Process
Sample Collection

Small blood samples obtained from captured birds

DNA Extraction

Using specialized kits to isolate genetic material

PCR Amplification

Nested PCR protocol targeting cytochrome b gene 2 6

Sequencing & Analysis

Comparing genetic sequences with known lineages 2

Essential Research Tools
Research Tool Specific Example Purpose in Research
Storage Medium Queen's lysis buffer, 96% ethanol, filter paper Preserves blood samples for future DNA analysis
DNA Extraction Kit DNeasy Blood and Tissue Kit Isolates genetic material from blood samples
Molecular Detection Nested PCR targeting cytochrome b gene Amplifies parasite DNA for detection
Morphological Identification Microscopic examination of blood smears Identifies parasites based on physical characteristics
Innovation: Feather-Based DNA

A 2023 study demonstrated that feather-based DNA could detect haemosporidian infections, offering a less invasive alternative to blood sampling 3 .

Innovation: Next-Gen Diagnostics

"Next-generation universal parasite diagnostics" (nUPDx) uses deep-amplicon sequencing to detect a broader range of parasites 7 .

Implications for Conservation and Human Health

Climate Change Impact

As climate change alters temperature and precipitation patterns, the distribution of both parasites and their vectors is shifting.

Research from the Colorado Rocky Mountains has demonstrated that higher elevations, once relatively free of these parasites, are becoming more suitable for transmission as temperatures warm 6 .

Public Health Connections

In Sri Lanka, which has eliminated human malaria but remains receptive to its reintroduction through imported cases, understanding avian Plasmodium parasites provides valuable insights for public health preparedness.

The country has developed sophisticated surveillance systems, including evaluating new diagnostic technologies like the Gazelle device, which detects malaria parasites through their magnetic properties 5 .

An Ongoing Scientific Journey

The investigation into Sri Lanka's avian blood parasites reveals a complex web of interactions between birds, parasites, and mosquitoes. Each discovery adds another piece to the puzzle of tropical ecosystem functioning.

As research continues, scientists are increasingly recognizing how these microscopic interactions influence broader ecological patterns, potentially affecting bird population dynamics, species distributions, and community composition.

References